Isolation and Characterization of Protoplasts from Saccharomyces rouxii

Cells of the osmotolerant yeast Saccharomyces rouxii were transformed to protoplasts in good yield (85%) by digesting cell walls with snail-gut enzyme in the presence of 10 mM dithioerythritol, 0.1 M sodium phosphate buffer (pH 6.8), and 2.0 M KCl. The requirement for 2.0 M KCl compares with that fo...

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Detalhes bibliográficos

Publicado no:	J Bacteriol
Principais autores:	Arnold, Wilfred N., Garrison, Robert G.
Formato:	Artigo
Idioma:	Inglês
Publicado em:	American Society for Microbiology (ASM) 1979
Assuntos:	Morphology and Ultrastructure
Acesso em linha:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC218323/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/438120/ https://ncbi.nlm.nih.govhttps://doi.org/10.1128/jb.137.3.1386-1394.1979
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