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Real time detection of DNA⋅RNA hybridization in living cells

Demonstrating hybridization between an antisense oligodeoxynucleotide and its mRNA target has proven to be extremely difficult in living cells. To address this fundamental problem in antisense research, we synthesized “molecular beacon” (MB) reporter oligodeoxynucleotides with matched fluorescent do...

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Autori principali: Sokol, Deborah L., Zhang, Xiaolin, Lu, Ponzy, Gewirtz, Alan M.
Natura: Artigo
Lingua:Inglês
Pubblicazione: National Academy of Sciences 1998
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Accesso online:https://ncbi.nlm.nih.gov/pmc/articles/PMC21676/
https://ncbi.nlm.nih.gov/pubmed/9751701
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