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Construction and validation of yeast artificial chromosome contig maps by RecA-assisted restriction endonuclease cleavage
RecA-assisted restriction endonuclease (RARE) cleavage is an “Achilles’ heel” approach to restriction mapping whereby a RecA-protein–oligodeoxynucleotide complex protects an individual restriction site from methylation, thus limiting subsequent digestion to a single, predetermined site. We have used...
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| Main Authors: | , , |
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| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado: |
The National Academy of Sciences
1998
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| Assuntos: | |
| Acceso en liña: | https://ncbi.nlm.nih.gov/pmc/articles/PMC21640/ https://ncbi.nlm.nih.gov/pubmed/9736734 |
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