RecA-AC: single-site cleavage of plasmids and chromosomes at any predetermined restriction site.

We have developed a novel version of the Achilles' Cleavage (AC) reaction in which virtually any restriction site on DNA of any size can be converted to a unique cleavage site. We first polymerized RecA protein on a synthetic oligodeoxyribonucleotide (oligo) in the presence of a nonhydrolyzable...

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Detaylı Bibliyografya
Asıl Yazarlar: Koob, M, Burkiewicz, A, Kur, J, Szybalski, W
Materyal Türü: Artigo
Dil:Inglês
Baskı/Yayın Bilgisi: 1992
Online Erişim:https://ncbi.nlm.nih.gov/pmc/articles/PMC334423/
https://ncbi.nlm.nih.gov/pubmed/1454542
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