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RecA-AC: single-site cleavage of plasmids and chromosomes at any predetermined restriction site.

We have developed a novel version of the Achilles' Cleavage (AC) reaction in which virtually any restriction site on DNA of any size can be converted to a unique cleavage site. We first polymerized RecA protein on a synthetic oligodeoxyribonucleotide (oligo) in the presence of a nonhydrolyzable...

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Bibliografische gegevens
Gepubliceerd in:Nucleic Acids Res
Hoofdauteurs: Koob, M, Burkiewicz, A, Kur, J, Szybalski, W
Formaat: Artigo
Taal:Inglês
Gepubliceerd in: Oxford University Press 1992
Online toegang:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC334423/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/1454542/
https://ncbi.nlm.nih.govhttps://doi.org/10.1093/nar/20.21.5831
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