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RecA-AC: single-site cleavage of plasmids and chromosomes at any predetermined restriction site.
We have developed a novel version of the Achilles' Cleavage (AC) reaction in which virtually any restriction site on DNA of any size can be converted to a unique cleavage site. We first polymerized RecA protein on a synthetic oligodeoxyribonucleotide (oligo) in the presence of a nonhydrolyzable...
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| Pubblicato in: | Nucleic Acids Res |
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| Autori principali: | , , , |
| Natura: | Artigo |
| Lingua: | Inglês |
| Pubblicazione: |
Oxford University Press
1992
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| Accesso online: | https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC334423/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/1454542/ https://ncbi.nlm.nih.govhttps://doi.org/10.1093/nar/20.21.5831 |
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