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RecA-AC: single-site cleavage of plasmids and chromosomes at any predetermined restriction site.
We have developed a novel version of the Achilles' Cleavage (AC) reaction in which virtually any restriction site on DNA of any size can be converted to a unique cleavage site. We first polymerized RecA protein on a synthetic oligodeoxyribonucleotide (oligo) in the presence of a nonhydrolyzable...
Sparad:
| Huvudupphovsmän: | , , , |
|---|---|
| Materialtyp: | Artigo |
| Språk: | Inglês |
| Publicerad: |
1992
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| Länkar: | https://ncbi.nlm.nih.gov/pmc/articles/PMC334423/ https://ncbi.nlm.nih.gov/pubmed/1454542 |
| Taggar: |
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