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Construction and validation of yeast artificial chromosome contig maps by RecA-assisted restriction endonuclease cleavage

RecA-assisted restriction endonuclease (RARE) cleavage is an “Achilles’ heel” approach to restriction mapping whereby a RecA-protein–oligodeoxynucleotide complex protects an individual restriction site from methylation, thus limiting subsequent digestion to a single, predetermined site. We have used...

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Main Authors: Lauer, Peter, Schneider, Sandra S., Gnirke, Andreas
格式: Artigo
語言:Inglês
出版: The National Academy of Sciences 1998
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在線閱讀:https://ncbi.nlm.nih.gov/pmc/articles/PMC21640/
https://ncbi.nlm.nih.gov/pubmed/9736734
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