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Molecular construction and characterization of nif mutants of the obligate methanotroph Methylosinus sp. strain 6.

We describe here a method for constructing mutants in bacteria that are not amenable to mutant isolation by conventional means. A one-step marker exchange procedure was used to construct nitrogen fixation (nif) mutants of the obligate methane-utilizing bacterium Methylosinus sp. strain 6, using tran...

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Pubblicato in:J Bacteriol
Autori principali: Toukdarian, A E, Lidstrom, M E
Natura: Artigo
Lingua:Inglês
Pubblicazione: American Society for Microbiology (ASM) 1984
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Accesso online:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC215363/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/6321452/
https://ncbi.nlm.nih.govhttps://doi.org/10.1128/jb.157.3.979-983.1984
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