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Molecular construction and characterization of nif mutants of the obligate methanotroph Methylosinus sp. strain 6.
We describe here a method for constructing mutants in bacteria that are not amenable to mutant isolation by conventional means. A one-step marker exchange procedure was used to construct nitrogen fixation (nif) mutants of the obligate methane-utilizing bacterium Methylosinus sp. strain 6, using tran...
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| Autori principali: | , |
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| Natura: | Artigo |
| Lingua: | Inglês |
| Pubblicazione: |
1984
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| Soggetti: | |
| Accesso online: | https://ncbi.nlm.nih.gov/pmc/articles/PMC215363/ https://ncbi.nlm.nih.gov/pubmed/6321452 |
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