Molecular construction and characterization of nif mutants of the obligate methanotroph Methylosinus sp. strain 6.
We describe here a method for constructing mutants in bacteria that are not amenable to mutant isolation by conventional means. A one-step marker exchange procedure was used to construct nitrogen fixation (nif) mutants of the obligate methane-utilizing bacterium Methylosinus sp. strain 6, using tran...
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| Publicado en: | J Bacteriol |
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| Autores principales: | , |
| Formato: | Artigo |
| Lenguaje: | Inglês |
| Publicado: |
American Society for Microbiology (ASM)
1984
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| Materias: | |
| Acceso en línea: | https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC215363/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/6321452/ https://ncbi.nlm.nih.govhttps://doi.org/10.1128/jb.157.3.979-983.1984 |
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