Energetic analysis of an antigen/antibody interface: alanine scanning mutagenesis and double mutant cycles on the HyHEL-10/lysozyme interaction.

Alanine scanning mutagenesis of the HyHEL-10 paratope of the HyHEL-10/HEWL complex demonstrates that the energetically important side chains (hot spots) of both partners are in contact. A plot of deltadeltaG(HyHEL-10_mutant) vs. deltadeltaG(HEWL_mutant) for the five of six interacting side-chain hyd...

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Bibliographic Details
Main Authors: Pons, J., Rajpal, A., Kirsch, J. F.
Format: Artigo
Language:Inglês
Published: Cold Spring Harbor Laboratory Press 1999
Subjects:
Research Article
Online Access:https://ncbi.nlm.nih.gov/pmc/articles/PMC2144329/
https://ncbi.nlm.nih.gov/pubmed/10338006
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