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Robustness of protein folding kinetics to surface hydrophobic substitutions.

We use both combinatorial and site-directed mutagenesis to explore the consequences of surface hydrophobic substitutions for the folding of two small single domain proteins, the src SH3 domain, and the IgG binding domain of Peptostreptococcal protein L. We find that in almost every case, destabilizi...

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Autores principales: Gu, H., Doshi, N., Kim, D. E., Simons, K. T., Santiago, J. V., Nauli, S., Baker, D.
Formato: Artigo
Lenguaje:Inglês
Publicado: Cold Spring Harbor Laboratory Press 1999
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Acceso en línea:https://ncbi.nlm.nih.gov/pmc/articles/PMC2144221/
https://ncbi.nlm.nih.gov/pubmed/10631990
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