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Surface point mutations that significantly alter the structure and stability of a protein's denatured state.

Significantly different m values (1.9-2.7 kcal mol-1 M-1) were observed for point mutations at a single, solvent-exposed site (T53) in a variant of the B1 domain of streptococcal Protein G using guanidine hydrochloride (GuHCl) as a denaturant. This report focuses on elucidating the energetic and str...

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Autores principales: Smith, C. K., Bu, Z., Anderson, K. S., Sturtevant, J. M., Engelman, D. M., Regan, L.
Formato: Artigo
Lenguaje:Inglês
Publicado: Cold Spring Harbor Laboratory Press 1996
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Acceso en línea:https://ncbi.nlm.nih.gov/pmc/articles/PMC2143264/
https://ncbi.nlm.nih.gov/pubmed/8897601
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