In vivo and in vitro synthesis of Escherichia coli maltose-binding protein under regulatory control of the lacUV5 promoter-operator.
It has not been possible to obtain in vitro expression of the positively regulated malE gene encoding the periplasmic maltose-binding protein (MBP) of Escherichia coli. To facilitate in vitro malE expression, we constructed plasmids that place the malE gene under transcriptional control of the lacUV...
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| Published in: | J Bacteriol |
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| Main Authors: | , , , |
| Format: | Artigo |
| Language: | Inglês |
| Published: |
American Society for Microbiology (ASM)
1985
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| Subjects: | |
| Online Access: | https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC214304/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/3902794/ https://ncbi.nlm.nih.govhttps://doi.org/10.1128/jb.164.2.665-673.1985 |
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