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In vivo and in vitro synthesis of Escherichia coli maltose-binding protein under regulatory control of the lacUV5 promoter-operator.

It has not been possible to obtain in vitro expression of the positively regulated malE gene encoding the periplasmic maltose-binding protein (MBP) of Escherichia coli. To facilitate in vitro malE expression, we constructed plasmids that place the malE gene under transcriptional control of the lacUV...

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Detalles Bibliográficos
Publicado en:J Bacteriol
Principais autores: Rasmussen, B A, MacGregor, C H, Ray, P H, Bassford, P J
Formato: Artigo
Idioma:Inglês
Publicado: American Society for Microbiology (ASM) 1985
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Acceso en liña:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC214304/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/3902794/
https://ncbi.nlm.nih.govhttps://doi.org/10.1128/jb.164.2.665-673.1985
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