In vivo and in vitro synthesis of Escherichia coli maltose-binding protein under regulatory control of the lacUV5 promoter-operator.

It has not been possible to obtain in vitro expression of the positively regulated malE gene encoding the periplasmic maltose-binding protein (MBP) of Escherichia coli. To facilitate in vitro malE expression, we constructed plasmids that place the malE gene under transcriptional control of the lacUV...

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Autors principals: Rasmussen, B A, MacGregor, C H, Ray, P H, Bassford, P J
Format: Artigo
Idioma:Inglês
Publicat: 1985
Matèries:
Research Article
Accés en línia:https://ncbi.nlm.nih.gov/pmc/articles/PMC214304/
https://ncbi.nlm.nih.gov/pubmed/3902794
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