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Identification of the catalytic histidine residue participating in the charge-relay system of carboxypeptidase Y.

The essential histidine residue of carboxypeptidase Y (CPY) was modified by a site-specific reagent, a chloromethylketone derivative of benzyloxycarbonyl-L-phenylalanine. The single modified histidine residue was converted to N tau-carboxy-methyl histidine (cmHis) upon performic acid oxidation. A pe...

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Autors principals: Jung, G., Ueno, H., Hayashi, R., Liao, T. H.
Format: Artigo
Idioma:Inglês
Publicat: Cold Spring Harbor Laboratory Press 1995
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Accés en línia:https://ncbi.nlm.nih.gov/pmc/articles/PMC2143012/
https://ncbi.nlm.nih.gov/pubmed/8563642
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