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Hydrophobic core repacking and aromatic-aromatic interaction in the thermostable mutant of T4 lysozyme Ser 117-->Phe.

The T4 lysozyme mutant Ser 117-->Phe was isolated fortuitously and found to be more thermostable than wild-type by 1.1-1.4 kcal/mol. In the wild-type structure, the side chain of Ser 117 is in a sterically restricted region near the protein surface and forms a short hydrogen bond with Asn 132. Th...

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Autori principali: Anderson, D. E., Hurley, J. H., Nicholson, H., Baase, W. A., Matthews, B. W.
Natura: Artigo
Lingua:Inglês
Pubblicazione: Cold Spring Harbor Laboratory Press 1993
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Accesso online:https://ncbi.nlm.nih.gov/pmc/articles/PMC2142442/
https://ncbi.nlm.nih.gov/pubmed/8401213
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