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Effect of pH and denaturants on the folding and stability of murine interleukin-6.

The conformation and stability of a recombinant mouse interleukin-6 (mIL-6) has been investigated by analytical ultracentrifugation, fluorescence spectroscopy, urea-gradient gel electrophoresis, and near- and far-ultraviolet circular dichroism. On decreasing the pH from 8.0 to 4.0, the tryptophan fl...

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Bibliografiske detaljer
Main Authors: Ward, L. D., Zhang, J. G., Checkley, G., Preston, B., Simpson, R. J.
Format: Artigo
Sprog:Inglês
Udgivet: Cold Spring Harbor Laboratory Press 1993
Fag:
Online adgang:https://ncbi.nlm.nih.gov/pmc/articles/PMC2142433/
https://ncbi.nlm.nih.gov/pubmed/8401214
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