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An Assay for Thiaminase I in Complex Biological Samples
An alternative method for measuring thiaminase I activity in complex samples is described. This assay is based on the selective consumption of the highly chromophoric 4-nitrothiophenolate by thiaminase I resulting in a large decrease in absorbance at 411 nm. This new assay is simple and sensitive an...
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| Main Authors: | , , |
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| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
2007
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC2140240/ https://ncbi.nlm.nih.gov/pubmed/17603991 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.ab.2007.06.001 |
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