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Cloning of genes that suppress an Escherichia coli K-12 alanine auxotroph when present in multicopy plasmids.

To facilitate molecular analyses of a previously uncharacterized gene involved in alanine synthesis, attempts were made to clone the wild-type allele of this gene, alaA, with a mini-Mu plasmid element used for in vivo cloning. Seventy-six independent Ala+ plasmids were isolated and characterized. Ph...

תיאור מלא

שמור ב:
מידע ביבליוגרפי
Main Authors: Wang, M D, Buckley, L, Berg, C M
פורמט: Artigo
שפה:Inglês
יצא לאור: 1987
נושאים:
גישה מקוונת:https://ncbi.nlm.nih.gov/pmc/articles/PMC214003/
https://ncbi.nlm.nih.gov/pubmed/2890623
תגים: הוספת תג
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