Cloning of genes that suppress an Escherichia coli K-12 alanine auxotroph when present in multicopy plasmids.
To facilitate molecular analyses of a previously uncharacterized gene involved in alanine synthesis, attempts were made to clone the wild-type allele of this gene, alaA, with a mini-Mu plasmid element used for in vivo cloning. Seventy-six independent Ala+ plasmids were isolated and characterized. Ph...
Guardat en:
| Publicat a: | J Bacteriol |
|---|---|
| Autors principals: | , , |
| Format: | Artigo |
| Idioma: | Inglês |
| Publicat: |
American Society for Microbiology (ASM)
1987
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| Matèries: | |
| Accés en línia: | https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC214003/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/2890623/ https://ncbi.nlm.nih.govhttps://doi.org/10.1128/jb.169.12.5610-5614.1987 |
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