Cloning of genes that suppress an Escherichia coli K-12 alanine auxotroph when present in multicopy plasmids.

To facilitate molecular analyses of a previously uncharacterized gene involved in alanine synthesis, attempts were made to clone the wild-type allele of this gene, alaA, with a mini-Mu plasmid element used for in vivo cloning. Seventy-six independent Ala+ plasmids were isolated and characterized. Ph...

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Veröffentlicht in:	J Bacteriol
Hauptverfasser:	Wang, M D, Buckley, L, Berg, C M
Format:	Artigo
Sprache:	Inglês
Veröffentlicht:	American Society for Microbiology (ASM) 1987
Schlagworte:	Research Article
Online-Zugang:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC214003/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/2890623/ https://ncbi.nlm.nih.govhttps://doi.org/10.1128/jb.169.12.5610-5614.1987
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