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Reconstitution of an active lactose carrier in vivo by simultaneous synthesis of two complementary protein fragments.

Escherichia coli lactose permease mediates the proton-driven translocation of galactosides across the cytoplasmic membrane. To define regions important for membrane insertion as well as for biological function, we constructed plasmids encoding different portions of the lactose carrier. Among several...

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Dettagli Bibliografici
Pubblicato in:J Bacteriol
Autori principali: Wrubel, W, Stochaj, U, Sonnewald, U, Theres, C, Ehring, R
Natura: Artigo
Lingua:Inglês
Pubblicazione: American Society for Microbiology (ASM) 1990
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Accesso online:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC213202/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/2203750/
https://ncbi.nlm.nih.govhttps://doi.org/10.1128/jb.172.9.5374-5381.1990
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