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Evidence for Internal and External Binding Sites on Human Tear Lipocalin
8-anilino-1-naphthalenesulfonic acid (ANS) is widely used as a probe for locating binding sites of proteins. To characterize the binding sites of tear lipocalin (TL), we studied ANS binding to apoTL by steady-state and time-resolved fluorescence. Deconvolution of ANS binding revealed that two lifeti...
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Main Authors: | , , |
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Formato: | Artigo |
Idioma: | Inglês |
Publicado em: |
2007
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Assuntos: | |
Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC2129106/ https://ncbi.nlm.nih.gov/pubmed/17945179 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.abb.2007.09.011 |
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