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Improved generation of recombinant baculovirus genomes in Escherichia coli

An improved method for the generation of recombinant baculoviruses by Tn7-mediated transposition is described. The method is based on the modified donor vector (pBVboost) and an improved selection scheme of the baculovirus bacmids in Escherichia coli with a mutated SacB gene. Recombinant bacmids can...

Whakaahuatanga katoa

I tiakina i:
Ngā taipitopito rārangi puna kōrero
I whakaputaina i:Nucleic Acids Res
Ngā kaituhi matua: Airenne, Kari J., Peltomaa, Erik, Hytönen, Vesa P., Laitinen, Olli H., Ylä-Herttuala, Seppo
Hōputu: Artigo
Reo:Inglês
I whakaputaina: Oxford University Press 2003
Ngā marau:
Urunga tuihono:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC212821/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/12930975/
https://ncbi.nlm.nih.govhttps://doi.org/10.1093/nar/gng102
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