Improved generation of recombinant baculovirus genomes in Escherichia coli
An improved method for the generation of recombinant baculoviruses by Tn7-mediated transposition is described. The method is based on the modified donor vector (pBVboost) and an improved selection scheme of the baculovirus bacmids in Escherichia coli with a mutated SacB gene. Recombinant bacmids can...
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| Publié dans: | Nucleic Acids Res |
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| Auteurs principaux: | , , , , |
| Format: | Artigo |
| Langue: | Inglês |
| Publié: |
Oxford University Press
2003
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| Sujets: | |
| Accès en ligne: | https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC212821/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/12930975/ https://ncbi.nlm.nih.govhttps://doi.org/10.1093/nar/gng102 |
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