Identification of the Serratia marcescens hemolysin determinant by cloning into Escherichia coli.

A cosmid bank of Serratia marcescens was established from which DNA fragments were cloned into the plasmid pBR322, which conferred the chromosomally encoded hemolytic activity to Escherichia coli K-12. By transposon mutagenesis with Tn1000 and Tn5 IS50L::phoA (TnphoA), the coding region was assigned...

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Bibliografske podrobnosti
Main Authors: Braun, V, Neuss, B, Ruan, Y, Schiebel, E, Schöffler, H, Jander, G
Format: Artigo
Jezik:Inglês
Izdano: 1987
Teme:
Research Article
Online dostop:https://ncbi.nlm.nih.gov/pmc/articles/PMC212107/
https://ncbi.nlm.nih.gov/pubmed/2437098
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