Identification of the Serratia marcescens hemolysin determinant by cloning into Escherichia coli.

A cosmid bank of Serratia marcescens was established from which DNA fragments were cloned into the plasmid pBR322, which conferred the chromosomally encoded hemolytic activity to Escherichia coli K-12. By transposon mutagenesis with Tn1000 and Tn5 IS50L::phoA (TnphoA), the coding region was assigned...

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Detalles Bibliográficos

Publicado en:	J Bacteriol
Autores principales:	Braun, V, Neuss, B, Ruan, Y, Schiebel, E, Schöffler, H, Jander, G
Formato:	Artigo
Lenguaje:	Inglês
Publicado:	American Society for Microbiology (ASM) 1987
Materias:	Research Article
Acceso en línea:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC212107/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/2437098/ https://ncbi.nlm.nih.govhttps://doi.org/10.1128/jb.169.5.2113-2120.1987
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