Identification of the Serratia marcescens hemolysin determinant by cloning into Escherichia coli.
A cosmid bank of Serratia marcescens was established from which DNA fragments were cloned into the plasmid pBR322, which conferred the chromosomally encoded hemolytic activity to Escherichia coli K-12. By transposon mutagenesis with Tn1000 and Tn5 IS50L::phoA (TnphoA), the coding region was assigned...
Salvato in:
| Pubblicato in: | J Bacteriol |
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| Autori principali: | , , , , , |
| Natura: | Artigo |
| Lingua: | Inglês |
| Pubblicazione: |
American Society for Microbiology (ASM)
1987
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| Soggetti: | |
| Accesso online: | https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC212107/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/2437098/ https://ncbi.nlm.nih.govhttps://doi.org/10.1128/jb.169.5.2113-2120.1987 |
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