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Reconstitution of protein translocation from solubilized yeast membranes reveals topologically distinct roles for BiP and cytosolic Hsc70
We reconstituted prepro-alpha-factor translocation and signal peptide processing using a yeast microsomal detergent soluble fraction formed into vesicles with soybean phospholipids. Reconstituted translocation required ATP, and was deficient when sec63 and kar2 (BiP) mutant cells were used as a sour...
Tallennettuna:
| Aineistotyyppi: | Artigo |
|---|---|
| Kieli: | Inglês |
| Julkaistu: |
The Rockefeller University Press
1993
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| Aiheet: | |
| Linkit: | https://ncbi.nlm.nih.gov/pmc/articles/PMC2119491/ https://ncbi.nlm.nih.gov/pubmed/8416998 |
| Tagit: |
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