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Reconstitution of protein translocation from solubilized yeast membranes reveals topologically distinct roles for BiP and cytosolic Hsc70
We reconstituted prepro-alpha-factor translocation and signal peptide processing using a yeast microsomal detergent soluble fraction formed into vesicles with soybean phospholipids. Reconstituted translocation required ATP, and was deficient when sec63 and kar2 (BiP) mutant cells were used as a sour...
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| Natura: | Artigo |
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| Lingua: | Inglês |
| Pubblicazione: |
The Rockefeller University Press
1993
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| Accesso online: | https://ncbi.nlm.nih.gov/pmc/articles/PMC2119491/ https://ncbi.nlm.nih.gov/pubmed/8416998 |
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