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Plasmid marker rescue transformation proceeds by breakage-reunion in Bacillus subtilis.

Bacillus subtilis carrying a plasmid which replicates with a copy number of about 1 was transformed with linearized homologous plasmid DNA labeled with the heavy isotopes 2H and 15N, in the presence of 32Pi and 6-(p-hydroxyphenylazo)-uracil to inhibit DNA replication. Plasmid DNA was isolated from t...

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Detalles Bibliográficos
Publicado en:J Bacteriol
Autores principales: Weinrauch, Y, Dubnau, D
Formato: Artigo
Lenguaje:Inglês
Publicado: American Society for Microbiology (ASM) 1987
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Acceso en línea:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC211920/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/3029025/
https://ncbi.nlm.nih.govhttps://doi.org/10.1128/jb.169.3.1205-1211.1987
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