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Plasmid marker rescue transformation proceeds by breakage-reunion in Bacillus subtilis.

Bacillus subtilis carrying a plasmid which replicates with a copy number of about 1 was transformed with linearized homologous plasmid DNA labeled with the heavy isotopes 2H and 15N, in the presence of 32Pi and 6-(p-hydroxyphenylazo)-uracil to inhibit DNA replication. Plasmid DNA was isolated from t...

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Bibliographic Details
Main Authors: Weinrauch, Y, Dubnau, D
Format: Artigo
Language:Inglês
Published: 1987
Subjects:
Online Access:https://ncbi.nlm.nih.gov/pmc/articles/PMC211920/
https://ncbi.nlm.nih.gov/pubmed/3029025
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