Plasmid marker rescue transformation proceeds by breakage-reunion in Bacillus subtilis.

Bacillus subtilis carrying a plasmid which replicates with a copy number of about 1 was transformed with linearized homologous plasmid DNA labeled with the heavy isotopes 2H and 15N, in the presence of 32Pi and 6-(p-hydroxyphenylazo)-uracil to inhibit DNA replication. Plasmid DNA was isolated from t...

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Pubblicato in:	J Bacteriol
Autori principali:	Weinrauch, Y, Dubnau, D
Natura:	Artigo
Lingua:	Inglês
Pubblicazione:	American Society for Microbiology (ASM) 1987
Soggetti:	Research Article
Accesso online:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC211920/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/3029025/ https://ncbi.nlm.nih.govhttps://doi.org/10.1128/jb.169.3.1205-1211.1987
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