Structural analysis of the Escherichia coli K-12 hisT operon by using a kanamycin resistance cassette.
We constructed a series of recombinant plasmids containing a kanamycin resistance (Kmr) cassette upstream from, within, and downstream from hisT, which encodes the tRNA modification enzyme pseudouridine synthase I. These Kmr insertions were then crossed directly into the bacterial chromosome. We det...
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| Publicado no: | J Bacteriol |
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| Principais autores: | , |
| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
American Society for Microbiology (ASM)
1987
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC211901/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/3029016/ https://ncbi.nlm.nih.govhttps://doi.org/10.1128/jb.169.3.1061-1070.1987 |
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