Structural analysis of the Escherichia coli K-12 hisT operon by using a kanamycin resistance cassette.
We constructed a series of recombinant plasmids containing a kanamycin resistance (Kmr) cassette upstream from, within, and downstream from hisT, which encodes the tRNA modification enzyme pseudouridine synthase I. These Kmr insertions were then crossed directly into the bacterial chromosome. We det...
Guardat en:
| Publicat a: | J Bacteriol |
|---|---|
| Autors principals: | , |
| Format: | Artigo |
| Idioma: | Inglês |
| Publicat: |
American Society for Microbiology (ASM)
1987
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| Matèries: | |
| Accés en línia: | https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC211901/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/3029016/ https://ncbi.nlm.nih.govhttps://doi.org/10.1128/jb.169.3.1061-1070.1987 |
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