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Structural analysis of the Escherichia coli K-12 hisT operon by using a kanamycin resistance cassette.

We constructed a series of recombinant plasmids containing a kanamycin resistance (Kmr) cassette upstream from, within, and downstream from hisT, which encodes the tRNA modification enzyme pseudouridine synthase I. These Kmr insertions were then crossed directly into the bacterial chromosome. We det...

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Udgivet i:J Bacteriol
Principais autores: Arps, P J, Winkler, M E
Format: Artigo
Sprog:Inglês
Udgivet: American Society for Microbiology (ASM) 1987
Fag:
Research Article
Online adgang:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC211901/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/3029016/
https://ncbi.nlm.nih.govhttps://doi.org/10.1128/jb.169.3.1061-1070.1987
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