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Expression of human plasma gelsolin in Escherichia coli and dissection of actin binding sites by segmental deletion mutagenesis
Human plasma gelsolin has been expressed in high yield and soluble form in Escherichia coli. The protein has nucleating and severing activities identical to those of plasma gelsolin and is fully calcium sensitive in its interactions with monomeric actin. A number of deletion mutants have been expres...
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| Format: | Artigo |
|---|---|
| Język: | Inglês |
| Wydane: |
The Rockefeller University Press
1989
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| Hasła przedmiotowe: | |
| Dostęp online: | https://ncbi.nlm.nih.gov/pmc/articles/PMC2115723/ https://ncbi.nlm.nih.gov/pubmed/2547804 |
| Etykiety: |
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