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Rate constants for the reactions of ATP- and ADP-actin with the ends of actin filaments
I measured the rate of elongation at the barbed and pointed ends of actin filaments by electron microscopy with Limulus sperm acrosomal processes as nuclei. With improvements in the mechanics of the assay, it was possible to measure growth rates from 0.05 to 280 s-1. At 22 degrees C in 1 mM MgCl2, 1...
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| Formato: | Artigo |
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| Idioma: | Inglês |
| Publicado em: |
The Rockefeller University Press
1986
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| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC2114620/ https://ncbi.nlm.nih.gov/pubmed/3793756 |
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