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In vitro insertion of leader peptidase into Escherichia coli membrane vesicles.

Leader peptidase is an integral protein of the Escherichia coli cytoplasmic membrane whose topology is known. We have taken advantage of this knowledge and available mutants of this enzyme to develop a genetic test for a cell-free protein translocation reaction. We report that leader peptidase inser...

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Bibliographic Details
Main Authors: Moore, K E, Dalbey, R E, Wickner, W
Format: Artigo
Language:Inglês
Published: 1988
Subjects:
Online Access:https://ncbi.nlm.nih.gov/pmc/articles/PMC211459/
https://ncbi.nlm.nih.gov/pubmed/3045096
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