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In vitro construction and characterization of phoA-lacZ gene fusions in Escherichia coli.

Using recombinant DNA techniques, we have constructed phoA-lacZ gene fusions. Two of the fusions encode hybrid proteins containing approximately half of alkaline phosphatase at the amino terminus joined to beta-galactosidase. For the one fusion strain analyzed in detail, it was shown that the hybrid...

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Detalhes bibliográficos
Publicado no:J Bacteriol
Principais autores: Michaelis, S, Guarente, L, Beckwith, J
Formato: Artigo
Idioma:Inglês
Publicado em: American Society for Microbiology (ASM) 1983
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC217467/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/6403507/
https://ncbi.nlm.nih.govhttps://doi.org/10.1128/jb.154.1.356-365.1983
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