Rapid topology mapping of Escherichia coli inner-membrane proteins by prediction and PhoA/GFP fusion analysis

We present an approach that allows rapid determination of the topology of Escherichia coli inner-membrane proteins by a combination of topology prediction and limited fusion-protein analysis. We derive new topology models for 12 inner-membrane proteins: MarC, PstA, TatC, YaeL, YcbM, YddQ, YdgE, YedZ...

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Detalhes bibliográficos
Main Authors: Drew, David, Sjöstrand, Dan, Nilsson, Johan, Urbig, Thomas, Chin, Chen-ni, de Gier, Jan-Willem, von Heijne, Gunnar
Formato: Artigo
Idioma:Inglês
Publicado em: The National Academy of Sciences 2002
Assuntos:
Biological Sciences
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC122409/
https://ncbi.nlm.nih.gov/pubmed/11867724
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1073/pnas.052018199
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