Rapid topology mapping of Escherichia coli inner-membrane proteins by prediction and PhoA/GFP fusion analysis

We present an approach that allows rapid determination of the topology of Escherichia coli inner-membrane proteins by a combination of topology prediction and limited fusion-protein analysis. We derive new topology models for 12 inner-membrane proteins: MarC, PstA, TatC, YaeL, YcbM, YddQ, YdgE, YedZ...

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Publié dans:	Proc Natl Acad Sci U S A
Auteurs principaux:	Drew, David, Sjöstrand, Dan, Nilsson, Johan, Urbig, Thomas, Chin, Chen-ni, de Gier, Jan-Willem, von Heijne, Gunnar
Format:	Artigo
Langue:	Inglês
Publié:	National Academy of Sciences 2002
Sujets:	Biological Sciences
Accès en ligne:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC122409/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/11867724/ https://ncbi.nlm.nih.govhttps://doi.org/10.1073/pnas.052018199
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