Construction of a dihydrofolate reductase-deficient mutant of Escherichia coli by gene replacement.

The dihydrofolate reductase (fol) gene in Escherichia coli has been deleted and replaced by a selectable marker. Verification of the delta fol::kan strain has been accomplished using genetic and biochemical criteria, including Southern analysis of the chromosomal DNA. The delta fol::kan mutation is...

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Detalhes bibliográficos

Publicado no:	J Bacteriol
Principais autores:	Howell, E E, Foster, P G, Foster, L M
Formato:	Artigo
Idioma:	Inglês
Publicado em:	American Society for Microbiology (ASM) 1988
Assuntos:	Research Article
Acesso em linha:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC211246/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/2838456/ https://ncbi.nlm.nih.govhttps://doi.org/10.1128/jb.170.7.3040-3045.1988
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