Construction of a dihydrofolate reductase-deficient mutant of Escherichia coli by gene replacement.
The dihydrofolate reductase (fol) gene in Escherichia coli has been deleted and replaced by a selectable marker. Verification of the delta fol::kan strain has been accomplished using genetic and biochemical criteria, including Southern analysis of the chromosomal DNA. The delta fol::kan mutation is...
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| Published in: | J Bacteriol |
|---|---|
| Main Authors: | , , |
| Format: | Artigo |
| Language: | Inglês |
| Published: |
American Society for Microbiology (ASM)
1988
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| Subjects: | |
| Online Access: | https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC211246/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/2838456/ https://ncbi.nlm.nih.govhttps://doi.org/10.1128/jb.170.7.3040-3045.1988 |
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