Construction of a fol mutant strain of Escherichia coli for use in dihydrofolate reductase mutagenesis experiments.
A strain of Escherichia coli with the fol gene deleted and a kan gene inserted in its place was created for use in cloning and isolation of mutant dihydrofolate reductase. Southern blot analysis and dihydrofolate reductase enzyme assays confirmed the delta fol::kan genotype. A thyA mutation accompan...
שמור ב:
| הוצא לאור ב: | J Bacteriol |
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| Principais autores: | , |
| פורמט: | Artigo |
| שפה: | Inglês |
| יצא לאור: |
American Society for Microbiology (ASM)
1988
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| נושאים: | |
| גישה מקוונת: | https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC211288/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/3290203/ https://ncbi.nlm.nih.govhttps://doi.org/10.1128/jb.170.7.3301-3304.1988 |
| תגים: |
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