Construction of a fol mutant strain of Escherichia coli for use in dihydrofolate reductase mutagenesis experiments.
A strain of Escherichia coli with the fol gene deleted and a kan gene inserted in its place was created for use in cloning and isolation of mutant dihydrofolate reductase. Southern blot analysis and dihydrofolate reductase enzyme assays confirmed the delta fol::kan genotype. A thyA mutation accompan...
Guardat en:
| Publicat a: | J Bacteriol |
|---|---|
| Autors principals: | , |
| Format: | Artigo |
| Idioma: | Inglês |
| Publicat: |
American Society for Microbiology (ASM)
1988
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| Matèries: | |
| Accés en línia: | https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC211288/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/3290203/ https://ncbi.nlm.nih.govhttps://doi.org/10.1128/jb.170.7.3301-3304.1988 |
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