High-efficiency transformation of bacterial cells by electroporation.
We have developed a method for efficiently generating transient pores in the outer membranes of Escherichia coli K-12 derivatives by using a new type of electroporation apparatus. The pores are large enough and persist long enough to facilitate the equilibration of plasmid molecules between the intr...
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| Publicado no: | J Bacteriol |
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| Principais autores: | , |
| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
American Society for Microbiology (ASM)
1988
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC211205/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/3286620/ https://ncbi.nlm.nih.govhttps://doi.org/10.1128/jb.170.6.2796-2801.1988 |
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