High-efficiency transformation of bacterial cells by electroporation.

We have developed a method for efficiently generating transient pores in the outer membranes of Escherichia coli K-12 derivatives by using a new type of electroporation apparatus. The pores are large enough and persist long enough to facilitate the equilibration of plasmid molecules between the intr...

Ausführliche Beschreibung

Bibliografische Detailangaben

Veröffentlicht in:	J Bacteriol
Hauptverfasser:	Calvin, N M, Hanawalt, P C
Format:	Artigo
Sprache:	Inglês
Veröffentlicht:	American Society for Microbiology (ASM) 1988
Schlagworte:	Research Article
Online-Zugang:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC211205/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/3286620/ https://ncbi.nlm.nih.govhttps://doi.org/10.1128/jb.170.6.2796-2801.1988
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