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High-efficiency transformation of bacterial cells by electroporation.

We have developed a method for efficiently generating transient pores in the outer membranes of Escherichia coli K-12 derivatives by using a new type of electroporation apparatus. The pores are large enough and persist long enough to facilitate the equilibration of plasmid molecules between the intr...

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Detalhes bibliográficos
Main Authors: Calvin, N M, Hanawalt, P C
Formato: Artigo
Idioma:Inglês
Publicado em: 1988
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC211205/
https://ncbi.nlm.nih.gov/pubmed/3286620
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