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Isolation of fla-lacZ fusions in Escherichia coli K-12: most fusions result in soluble beta-galactosidase.
A series of fusions of flagellar genes to the lacZ gene was generated by insertion of Mu dII301 (Apr lac) bacteriophage into the genome of Escherichia coli. The beta-galactosidase activity in each resulting mutant was measured, and the location of the activity in the membrane, periplasmic, or cytopl...
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| Materialtyp: | Artigo |
| Språk: | Inglês |
| Publicerad: |
1988
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| Länkar: | https://ncbi.nlm.nih.gov/pmc/articles/PMC211065/ https://ncbi.nlm.nih.gov/pubmed/3127385 |
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